4XTL

Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3

4XTL の概要

• エントリーDOI: 10.2210/pdb4xtl/pdb
• 分子名称: Sodium pumping rhodopsin, SODIUM ION, EICOSANE, ... (5 entities in total)
• 機能のキーワード: ion pump, membrane protein
• 由来する生物種: Dokdonia eikasta
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42692.78

構造登録者

Gushchin, I.,Shevchenko, V.,Polovinkin, V.,Gordeliy, V. (登録日: 2015-01-23, 公開日: 2015-04-01, 最終更新日: 2024-01-10)

主引用文献

Gushchin, I.,Shevchenko, V.,Polovinkin, V.,Kovalev, K.,Alekseev, A.,Round, E.,Borshchevskiy, V.,Balandin, T.,Popov, A.,Gensch, T.,Fahlke, C.,Bamann, C.,Willbold, D.,Buldt, G.,Bamberg, E.,Gordeliy, V.
Crystal structure of a light-driven sodium pump.
Nat.Struct.Mol.Biol., 22:390-395, 2015

PubMed Abstract: Recently, the first known light-driven sodium pumps, from the microbial rhodopsin family, were discovered. We have solved the structure of one of them, Krokinobacter eikastus rhodopsin 2 (KR2), in the monomeric blue state and in two pentameric red states, at resolutions of 1.45 Å and 2.2 and 2.8 Å, respectively. The structures reveal the ion-translocation pathway and show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal α-helix and that the ion-uptake cavity is unexpectedly large and open to the surface. Obstruction of the cavity with the mutation G263F imparts KR2 with the ability to pump potassium. These results pave the way for the understanding and rational design of cation pumps with new specific properties valuable for optogenetics.

PubMed: 25849142
DOI: 10.1038/nsmb.3002

実験手法

X-RAY DIFFRACTION (1.45 Å)