4XPJ

Crystal structure of Nerve growth factor in complex with lysophosphatidylinositol

4XPJ の概要

• エントリーDOI: 10.2210/pdb4xpj/pdb
• 分子名称: Beta-nerve growth factor, (2R)-2-hydroxy-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}propyl tridecanoate (3 entities in total)
• 機能のキーワード: nerve growth factor, lysopi, hormone
• 由来する生物種: Mus musculus (Mouse)
• 細胞内の位置: Secreted: P01139
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27513.05

構造登録者

Sun, H.L.,Jiang, T. (登録日: 2015-01-17, 公開日: 2015-07-15, 最終更新日: 2024-11-13)

主引用文献

Sun, H.L.,Jiang, T.
The structure of nerve growth factor in complex with lysophosphatidylinositol
Acta Crystallogr.,Sect.F, 71:906-912, 2015

PubMed Abstract: Nerve growth factor (NGF) is an important protein that is involved in a variety of physiological processes in cell survival, differentiation, proliferation and maintenance. The previously reported crystal structure of mouse NGF (mNGF) in complex with lysophosphatidylserine (LysoPS) showed that mNGF can bind LysoPS at its dimeric interface. To expand the understanding of the structural basis for specific lipid recognition by NGF, the crystal structure of mNGF complexed with lysophosphatidylinositol (13:0 LysoPI) was solved. Interestingly, in addition to Lys88, which interacts with the head glycerol group and the phosphate group of LysoPI, as seen in the mNGF-LysoPS structure, two additional residues, Tyr52 and Arg50, were found to assist in lipid binding by forming hydrogen bonds to the inositol moiety of the LysoPI molecule. The results suggest a specific recognition mechanism of inositol group-containing lipids by NGF, which may help in the design of bioactive compounds that can be delivered by NGF.

PubMed: 26144237
DOI: 10.1107/S2053230X15008870

実験手法

X-RAY DIFFRACTION (2.605 Å)