4XOS

ANP32A LRR domain

Summary for 4XOS

• Entry DOI: 10.2210/pdb4xos/pdb
• Descriptor: Acidic leucine-rich nuclear phosphoprotein 32 family member A, CHLORIDE ION, GLYCEROL, ... (4 entities in total)
• Functional Keywords: leucine rich repeat tumor suppressor, component of the set complex, antitumor protein
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus: P39687
• Total number of polymer chains: 2
• Total formula weight: 34800.47

Authors

Zamora-Caballero, S.,Bravo, J. (deposition date: 2015-01-16, release date: 2015-06-03, Last modification date: 2024-01-10)

Primary citation

Zamora-Caballero, S.,Siauciunaite-Gaubard, L.,Bravo, J.
High-resolution crystal structure of the leucine-rich repeat domain of the human tumour suppressor PP32A (ANP32A).
Acta Crystallogr.,Sect.F, 71:684-687, 2015

PubMed Abstract: Acidic leucine-rich nuclear phosphoprotein 32A (PP32A) is a tumour suppressor whose expression is altered in many cancers. It is an apoptotic enhancer that stimulates apoptosome-mediated caspase activation and also forms part of a complex involved in caspase-independent apoptosis (the SET complex). Crystals of a fragment of human PP32A corresponding to the leucine-rich repeat domain, a widespread motif suitable for protein-protein interactions, have been obtained. The structure has been refined to 1.56 Å resolution. This domain was previously solved at 2.4 and 2.69 Å resolution (PDB entries 2je0 and 2je1, respectively). The new high-resolution structure shows some differences from previous models: there is a small displacement in the turn connecting the first α-helix (α1) to the first β-strand (β1), which slightly changes the position of α1 in the structure. The shift in the turn is observed in the context of a new crystal packing unrelated to those of previous structures.

PubMed: 26057796
DOI: 10.1107/S2053230X15006457

Experimental method

X-RAY DIFFRACTION (1.559 Å)