4XIU

Binary complex structure of Klenow fragment of Taq DNA polymerase I707L mutant with DNA containing TTT overhang

Summary for 4XIU

Entry DOI	10.2210/pdb4xiu/pdb
Descriptor	Synthetic oligonucleotide primer strand, Synthetic oligonucleotide template strand, DNA polymerase I, thermostable, ... (5 entities in total)
Functional Keywords	protein-dna complex, transferase-dna complex, transferase/dna
Biological source	Thermus aquaticus More
Total number of polymer chains	3
Total formula weight	68836.35
Authors	Wu, E.Y. (deposition date: 2015-01-07, release date: 2015-01-21, Last modification date: 2023-09-27)
Primary citation	Wu, E.Y.,Walsh, A.R.,Materne, E.C.,Hiltner, E.P.,Zielinski, B.,Miller, B.R.,Mawby, L.,Modeste, E.,Parish, C.A.,Barnes, W.M.,Kermekchiev, M.B. A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase. Biochemistry, 54:881-889, 2015 Cited by PubMed: 25537790 DOI: 10.1021/bi501198f PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation