4XAU

Crystal structure of AtS13 from Actinomadura melliaura

4XAU の概要

• エントリーDOI: 10.2210/pdb4xau/pdb
• 関連するPDBエントリー: 4RXK
• 分子名称: Putative aminotransferase, PYRIDOXAL-5'-PHOSPHATE (3 entities in total)
• 機能のキーワード: sugar aminotransferase, structural genomics, psi-2, protein structure initiative, enzyme discovery for natural product biosynthesis, natpro, transferase
• 由来する生物種: Actinomadura melliaura
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 302863.27

構造登録者

Wang, F.,Singh, S.,Xu, W.,Thorson, J.S.,Phillips Jr., G.N.,Enzyme Discovery for Natural Product Biosynthesis (NatPro) (登録日: 2014-12-15, 公開日: 2014-12-24, 最終更新日: 2023-09-27)

主引用文献

Singh, S.,Kim, Y.,Wang, F.,Bigelow, L.,Endres, M.,Kharel, M.K.,Babnigg, G.,Bingman, C.A.,Joachimiak, A.,Thorson, J.S.,Phillips, G.N.
Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis.
Proteins, 83:1547-1554, 2015

PubMed Abstract: AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.

PubMed: 26061967
DOI: 10.1002/prot.24844

実験手法

X-RAY DIFFRACTION (3.0012 Å)