4X3R

Avi-GCPII structure in complex with FITC-conjugated GCPII-specific inhibitor

4X3R の概要

• エントリーDOI: 10.2210/pdb4x3r/pdb
• 分子名称: Glutamate carboxypeptidase 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: glycoprotein, metallopeptidase, inhibitor, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane ; Single-pass type II membrane protein . Isoform PSMA': Cytoplasm: Q04609
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 87653.81

構造登録者

Tykvart, J.,Konvalinka, J. (登録日: 2014-12-01, 公開日: 2015-10-14, 最終更新日: 2024-11-06)

主引用文献

Tykvart, J.,Schimer, J.,Jancarik, A.,Barinkova, J.,Navratil, V.,Starkova, J.,Sramkova, K.,Konvalinka, J.,Majer, P.,Sacha, P.
Design of highly potent urea-based, exosite-binding inhibitors selective for glutamate carboxypeptidase II.
J.Med.Chem., 58:4357-4363, 2015

PubMed Abstract: We present here a structure-aided design of inhibitors targeting the active site as well as exosites of glutamate carboxypeptidase II (GCPII), a prostate cancer marker, preparing potent and selective inhibitors that are more than 1000-fold more active toward GCPII than its closest human homologue, glutamate carboxypeptidase III (GCPIII). Additionally, we demonstrate that the prepared inhibitor conjugate can be used for sensitive and selective imaging of GCPII in mammalian cells.

PubMed: 25923815
DOI: 10.1021/acs.jmedchem.5b00278

実験手法

X-RAY DIFFRACTION (1.86 Å)