4X3B

A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering

4X3B の概要

• エントリーDOI: 10.2210/pdb4x3b/pdb
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: lysozyme microcrystals, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14566.87

構造登録者

Roedig, P.,Vartiainen, I.,Duman, R.,Panneerselvam, S.,Stuebe, N.,Lorbeer, O.,Warmer, M.,Sutton, G.,Stuart, D.I.,Weckert, E.,David, C.,Wagner, A.,Meents, A. (登録日: 2014-11-28, 公開日: 2015-06-10, 最終更新日: 2024-10-23)

主引用文献

Roedig, P.,Vartiainen, I.,Duman, R.,Panneerselvam, S.,Stube, N.,Lorbeer, O.,Warmer, M.,Sutton, G.,Stuart, D.I.,Weckert, E.,David, C.,Wagner, A.,Meents, A.
A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering.
Sci Rep, 5:10451-10451, 2015

PubMed Abstract: At low emittance synchrotron sources it has become possible to perform structure determinations from the measurement of multiple microcrystals which were previously considered too small for diffraction experiments. Conventional mounting techniques do not fulfill the requirements of these new experiments. They significantly contribute to background scattering and it is difficult to locate the crystals, making them incompatible with automated serial crystallography. We have developed a micro-fabricated sample holder from single crystalline silicon with micropores, which carries up to thousands of crystals and significantly reduces the background scattering level. For loading, the suspended microcrystals are pipetted onto the chip and excess mother liquor is subsequently soaked off through the micropores. Crystals larger than the pore size are retained and arrange themselves according to the micropore pattern. Using our chip we were able to collect 1.5 Å high resolution diffraction data from protein microcrystals with sizes of 4 micrometers and smaller.

PubMed: 26022615
DOI: 10.1038/srep10451

実験手法

X-RAY DIFFRACTION (2.1 Å)