4X0G

Structure of Bsg25A binding with DNA

4X0G の概要

• エントリーDOI: 10.2210/pdb4x0g/pdb
• 分子名称: Blastoderm-specific gene 25A, DNA (5'-D(*GP*TP*TP*CP*CP*AP*AP*TP*TP*GP*GP*AP*A)-3'), ACETATE ION, ... (4 entities in total)
• 機能のキーワード: bsg25a, elba1, ben. dna-binding, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 64692.07

構造登録者

Ren, A. (登録日: 2014-11-21, 公開日: 2015-01-21, 最終更新日: 2023-09-27)

主引用文献

Dai, Q.,Ren, A.,Westholm, J.O.,Duan, H.,Patel, D.J.,Lai, E.C.
Common and distinct DNA-binding and regulatory activities of the BEN-solo transcription factor family.
Genes Dev., 29:48-62, 2015

PubMed Abstract: Recently, the BEN (BANP, E5R, and NAC1) domain was recognized as a new class of conserved DNA-binding domain. The fly genome encodes three proteins that bear only a single BEN domain ("BEN-solo" factors); namely, Insensitive (Insv), Bsg25A (Elba1), and CG9883 (Elba2). Insv homodimers preferentially bind CCAATTGG palindromes throughout the genome to mediate transcriptional repression, whereas Bsg25A and Elba2 heterotrimerize with their obligate adaptor, Elba3 (i.e., the ELBA complex), to recognize a CCAATAAG motif in the Fab-7 insulator. While these data suggest distinct DNA-binding properties of BEN-solo proteins, we performed reporter assays that indicate that both Bsg25A and Elba2 can individually recognize Insv consensus sites efficiently. We confirmed this by solving the structure of Bsg25A complexed to the Insv site, which showed that key aspects of the BEN:DNA recognition strategy are similar between these proteins. We next show that both Insv and ELBA proteins are competent to mediate transcriptional repression via Insv consensus sequences but that the ELBA complex appears to be selective for the ELBA site. Reciprocally, genome-wide analysis reveals that Insv exhibits significant cobinding to class I insulator elements, indicating that it may also contribute to insulator function. Indeed, we observed abundant Insv binding within the Hox complexes with substantial overlaps with class I insulators, many of which bear Insv consensus sites. Moreover, Insv coimmunoprecipitates with the class I insulator factor CP190. Finally, we observed that Insv harbors exclusive activity among fly BEN-solo factors with respect to regulation of Notch-mediated cell fate choices in the peripheral nervous system. This in vivo activity is recapitulated by BEND6, a mammalian BEN-solo factor that conserves the Notch corepressor function of Insv but not its capacity to bind Insv consensus sites. Altogether, our data define an array of common and distinct biochemical and functional properties of this new family of transcription factors.

PubMed: 25561495
DOI: 10.1101/gad.252122.114

実験手法

X-RAY DIFFRACTION (3.2065 Å)