4X0B
Structure of tRNA-processing enzyme complex 7
Summary for 4X0B
| Entry DOI | 10.2210/pdb4x0b/pdb |
| Related | 4WC2 4X0A |
| Descriptor | Poly A polymerase, RNA (77-MER) (2 entities in total) |
| Functional Keywords | rna nucleotidyltransferase, cca-adding enzyme, a-adding enzyme, transferase-rna complex, transferase/rna |
| Biological source | Aquifex aeolicus More |
| Total number of polymer chains | 2 |
| Total formula weight | 71356.45 |
| Authors | Yamashita, S.,Tomita, K. (deposition date: 2014-11-21, release date: 2015-04-15, Last modification date: 2023-11-08) |
| Primary citation | Yamashita, S.,Martinez, A.,Tomita, K. Measurement of Acceptor-T Psi C Helix Length of tRNA for Terminal A76-Addition by A-Adding Enzyme. Structure, 23:830-842, 2015 Cited by PubMed Abstract: The 3'-terminal CCA (C74C75A76-3') of tRNA is required for protein synthesis. In Aquifex aeolicus, the CCA-3' is synthesized by CC-adding and A-adding enzymes, although in most organisms, CCA is synthesized by a single CCA-adding enzyme. The mechanisms by which the A-adding enzyme adds only A76, but not C74C75, onto tRNA remained elusive. The complex structures of the enzyme with various tRNAs revealed the presence of a single tRNA binding site on the enzyme, with the enzyme measuring the acceptor-TΨC helix length of tRNA. The 3'-C75 of tRNA lacking A76 can reach the active site and the size and shape of the nucleotide binding pocket at the insertion stage are suitable for ATP. The 3'-C74 of tRNA lacking C75A76 cannot reach the active site, although CTP or ATP can bind the active pocket. Thus, the A-adding enzyme adds only A76, but not C74C75, onto tRNA. PubMed: 25914059DOI: 10.1016/j.str.2015.03.013 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
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