4WZX

ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins

4WZX の概要

• エントリーDOI: 10.2210/pdb4wzx/pdb
• 分子名称: Serine/threonine-protein kinase ULK3, IST1 homolog, COBALT (II) ION, ... (5 entities in total)
• 機能のキーワード: mit domain, ulk3, mim, ist1, escrt, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13442.08

構造登録者

Caballe, A.,Wenzel, D.M.,Agromayor, M.,Alam, S.L.,Skalicky, J.J.,Kloc, M.,Carlton, J.G.,Labrador, L.,Sundquist, W.I.,Martin-Serrano, J. (登録日: 2014-11-20, 公開日: 2015-06-03, 最終更新日: 2024-02-28)

主引用文献

Caballe, A.,Wenzel, D.M.,Agromayor, M.,Alam, S.L.,Skalicky, J.J.,Kloc, M.,Carlton, J.G.,Labrador, L.,Sundquist, W.I.,Martin-Serrano, J.
ULK3 regulates cytokinetic abscission by phosphorylating ESCRT-III proteins.
Elife, 4:e06547-e06547, 2015

PubMed Abstract: The endosomal sorting complexes required for transport (ESCRT) machinery mediates the physical separation between daughter cells during cytokinetic abscission. This process is regulated by the abscission checkpoint, a genome protection mechanism that relies on Aurora B and the ESCRT-III subunit CHMP4C to delay abscission in response to chromosome missegregation. In this study, we show that Unc-51-like kinase 3 (ULK3) phosphorylates and binds ESCRT-III subunits via tandem MIT domains, and thereby, delays abscission in response to lagging chromosomes, nuclear pore defects, and tension forces at the midbody. Our structural and biochemical studies reveal an unusually tight interaction between ULK3 and IST1, an ESCRT-III subunit required for abscission. We also demonstrate that IST1 phosphorylation by ULK3 is an essential signal required to sustain the abscission checkpoint and that ULK3 and CHMP4C are functionally linked components of the timer that controls abscission in multiple physiological situations.

PubMed: 26011858
DOI: 10.7554/eLife.06547

実験手法

X-RAY DIFFRACTION (1.3859 Å)