4WWU

Structure of Mex67:Mtr2

4WWU の概要

• エントリーDOI: 10.2210/pdb4wwu/pdb
• 分子名称: mRNA export factor MEX67, mRNA transport regulator MTR2, ZINC ION (3 entities in total)
• 機能のキーワード: mrna nuclear export factor, transport protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus: Q99257 P34232
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 526423.34

構造登録者

Aibara, S.,Valkov, E.,Stewart, M. (登録日: 2014-11-12, 公開日: 2015-02-04, 最終更新日: 2024-05-08)

主引用文献

Aibara, S.,Valkov, E.,Lamers, M.,Stewart, M.
Domain organization within the nuclear export factor Mex67:Mtr2 generates an extended mRNA binding surface.
Nucleic Acids Res., 43:1927-1936, 2015

PubMed Abstract: The Mex67:Mtr2 complex is the principal yeast nuclear export factor for bulk mRNA and also contributes to ribosomal subunit export. Mex67 is a modular protein constructed from four domains (RRM, LRR, NTF2-like and UBA) that have been thought to be joined by flexible linkers like beads on a string, with the RRM and LRR domains binding RNAs and the NTF2-like and UBA domains binding FG-nucleoporins to facilitate movement through nuclear pores. Here, we show that the NTF2-like domain from Saccharomyces cerevisiae Mex67:Mtr2 also contributes to RNA binding. Moreover, the 3.3 Å resolution crystal structure of the Mex67(ΔUBA):Mtr2 complex, supplemented with small angle X-ray scattering data, indicated that the LRR domain has a defined spatial relationship to the Mex67(NTF2L):Mtr2 region. Conversely, the RRM domain and especially the UBA domain are more mobile. The conformation assumed by the LRR and NTF2-like domains results in clusters of positively-charged residues on each becoming arranged to form a continuous interface for binding RNA on the opposite side of the complex to the region that interacts with FG-nucleoporins to facilitate passage through nuclear pores.

PubMed: 25618852
DOI: 10.1093/nar/gkv030

実験手法

X-RAY DIFFRACTION (3.301 Å)