4WWJ

UndA, an oxygen-activating, non-heme iron dependent desaturase/decarboxylase

4WWJ の概要

• エントリーDOI: 10.2210/pdb4wwj/pdb
• 関連するPDBエントリー: 4WWZ 4WX0
• 分子名称: TENA/THI-4 family protein, FE (III) ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: non-heme iron dependent desaturase/decarboxylase, 1-undecene biosynthesis, aliphatic medium-chain 1-alkenes biosynthesis, metalloenzyme, oxidoreductase
• 由来する生物種: Pseudomonas protegens Pf-5
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62416.37

構造登録者

Li, X.,Cate, J.D.H. (登録日: 2014-11-11, 公開日: 2014-12-24, 最終更新日: 2023-09-27)

主引用文献

Rui, Z.,Li, X.,Zhu, X.,Liu, J.,Domigan, B.,Barr, I.,Cate, J.H.,Zhang, W.
Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase.
Proc.Natl.Acad.Sci.USA, 111:18237-18242, 2014

PubMed Abstract: Aliphatic medium-chain 1-alkenes (MCAEs, ∼10 carbons) are "drop-in" compatible next-generation fuels and precursors to commodity chemicals. Mass production of MCAEs from renewable resources holds promise for mitigating dependence on fossil hydrocarbons. An MCAE, such as 1-undecene, is naturally produced by Pseudomonas as a semivolatile metabolite through an unknown biosynthetic pathway. We describe here the discovery of a single gene conserved in Pseudomonas responsible for 1-undecene biosynthesis. The encoded enzyme is able to convert medium-chain fatty acids (C10-C14) into their corresponding terminal olefins using an oxygen-activating, nonheme iron-dependent mechanism. Both biochemical and X-ray crystal structural analyses suggest an unusual mechanism of β-hydrogen abstraction during fatty acid substrate activation. Our discovery unveils previously unidentified chemistry in the nonheme Fe(II) enzyme family, provides an opportunity to explore the biology of 1-undecene in Pseudomonas, and paves the way for tailored bioconversion of renewable raw materials to MCAE-based biofuels and chemical commodities.

PubMed: 25489112
DOI: 10.1073/pnas.1419701112

実験手法

X-RAY DIFFRACTION (1.9 Å)