4WWD

High-resolution structure of the Co-bound form of the Y135F mutant of C. metallidurans CnrXs

4WWD の概要

• エントリーDOI: 10.2210/pdb4wwd/pdb
• 分子名称: Nickel and cobalt resistance protein CnrR, COBALT (II) ION, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: nickel sensor, metal binding protein
• 由来する生物種: Ralstonia metallidurans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13554.85

構造登録者

Volbeda, A.,Coves, J.,Maillard, A.P.,Kuennemann, S.,Grosse, C.,Schleuder, G.,Petit-Haertlein, I.,de Rosny, E.,Nies, D.H. (登録日: 2014-11-10, 公開日: 2015-02-11, 最終更新日: 2024-05-08)

主引用文献

Maillard, A.P.,Kunnemann, S.,Groe, C.,Volbeda, A.,Schleuder, G.,Petit-Hartlein, I.,de Rosny, E.,Nies, D.H.,Coves, J.
Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding.
Metallomics, 7:622-631, 2015

PubMed Abstract: Resistance to high concentration of nickel ions is mediated in Cupriavidus metallidurans by the CnrCBA transenvelope efflux complex. Expression of the cnrCBA genes is regulated by the transmembrane signal transduction complex CnrYXH. Together, the metal sensor CnrX and the transmembrane antisigma factor CnrY control the availability of the extracytoplasmic function sigma factor CnrH. Release of CnrH from sequestration by CnrY at the cytoplasmic side of the membrane depends essentially on the binding of the agonist metal ion Ni(ii) to the periplasmic metal sensor domain of CnrX. CnrH availability leads to transcription initiation at the promoters cnrYp and cnrCp and to the expression of the genes in the cnrYXHCBA nickel resistance determinant. The first steps of signal propagation by CnrX rely on subtle metal-dependent allosteric modifications. To study the nickel-mediated triggering process by CnrX, we have altered selected residues, F66, M123, and Y135, and explored the physiological consequences of these changes with respect to metal resistance, expression of a cnrCBA-lacZ reporter fusion and protein production. M123C- and Y135F-CnrXs have been further characterized in vitro by metal affinity measurements and crystallographic structure analysis. Atomic-resolution structures of metal-bound M123C- and Y135F-CnrXs showed that Ni(ii) binds two of the three canonical conformations identified and that Ni(ii) sensing likely proceeds by conformation selection.

PubMed: 25628016
DOI: 10.1039/c4mt00293h

実験手法

X-RAY DIFFRACTION (1.3 Å)