4WTA

CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH S15G E86Q E87Q C223H V321I MUTATIONS AND DELTA8 BETA HAIRPIN LOOP DELETION IN COMPLEX WITH UDP, MN2+ AND SYMMETRICAL PRIMER TEMPLATE 5'-CAAAAUUU

4WTA の概要

• エントリーDOI: 10.2210/pdb4wta/pdb
• 分子名称: RNA PRIMER TEMPLATE CAAAAUUU, RNA-directed RNA polymerase, MANGANESE (II) ION, ... (6 entities in total)
• 機能のキーワード: hcv, viral, ns5b, rdrp, resistance mutation, template, primer, primed initiation, delta8 beta hairpin loop deletion, transferase-rna complex, transferase/rna
• 由来する生物種: Hepatitis C virus JFH-1; 詳細
• 細胞内の位置: Core protein p21: Host endoplasmic reticulum membrane ; Single-pass membrane protein . Core protein p19: Virion . Envelope glycoprotein E1: Virion membrane ; Single-pass type I membrane protein . Envelope glycoprotein E2: Virion membrane ; Single-pass type I membrane protein . p7: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Protease NS2-3: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 5A: Host endoplasmic reticulum membrane ; Peripheral membrane protein . RNA-directed RNA polymerase: Host endoplasmic reticulum membrane ; Single-pass type I membrane protein : Q99IB8
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69350.73

構造登録者

Edwards, T.E.,Appleby, T.C. (登録日: 2014-10-29, 公開日: 2015-02-11, 最終更新日: 2023-09-27)

• 主引用文献: Appleby, T.C.,Perry, J.K.,Murakami, E.,Barauskas, O.,Feng, J.,Cho, A.,Fox, D.,Wetmore, D.R.,McGrath, M.E.,Ray, A.S.,Sofia, M.J.,Swaminathan, S.,Edwards, T.E.; Structural basis for RNA replication by the hepatitis C virus polymerase.; Science, 347:771-775, 2015; PubMed: 25678663; DOI: 10.1126/science.1259210

実験手法

X-RAY DIFFRACTION (2.8 Å)