4WMQ

Structure of Human Intelectin-1

4WMQ の概要

• エントリーDOI: 10.2210/pdb4wmq/pdb
• 関連するPDBエントリー: 4WMY
• 分子名称: Intelectin-1, CALCIUM ION (3 entities in total)
• 機能のキーワード: lectin, disulfide-linked, carbohydrate-binding, innate immunity, calcium, microbe-binding, microbe-specific, sugar binding protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane; Lipid-anchor, GPI-anchor: Q8WWA0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68757.50

構造登録者

Wangkanont, K.,Kiessling, L.L.,Forest, K.T. (登録日: 2014-10-09, 公開日: 2015-07-01, 最終更新日: 2024-11-20)

主引用文献

Wesener, D.A.,Wangkanont, K.,McBride, R.,Song, X.,Kraft, M.B.,Hodges, H.L.,Zarling, L.C.,Splain, R.A.,Smith, D.F.,Cummings, R.D.,Paulson, J.C.,Forest, K.T.,Kiessling, L.L.
Recognition of microbial glycans by human intelectin-1.
Nat.Struct.Mol.Biol., 22:603-610, 2015

PubMed Abstract: The glycans displayed on mammalian cells can differ markedly from those on microbes. Such differences could, in principle, be 'read' by carbohydrate-binding proteins, or lectins. We used glycan microarrays to show that human intelectin-1 (hIntL-1) does not bind known human glycan epitopes but does interact with multiple glycan epitopes found exclusively on microbes: β-linked D-galactofuranose (β-Galf), D-phosphoglycerol-modified glycans, heptoses, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). The 1.6-Å-resolution crystal structure of hIntL-1 complexed with β-Galf revealed that hIntL-1 uses a bound calcium ion to coordinate terminal exocyclic 1,2-diols. N-acetylneuraminic acid (Neu5Ac), a sialic acid widespread in human glycans, has an exocyclic 1,2-diol but does not bind hIntL-1, probably owing to unfavorable steric and electronic effects. hIntL-1 marks only Streptococcus pneumoniae serotypes that display surface glycans with terminal 1,2-diol groups. This ligand selectivity suggests that hIntL-1 functions in microbial surveillance.

PubMed: 26148048
DOI: 10.1038/nsmb.3053

実験手法

X-RAY DIFFRACTION (1.8 Å)