4WLA

Time Resolved Serial Femtosecond Crystallography Captures High Resolution Intermediates of PYP

4WLA の概要

• エントリーDOI: 10.2210/pdb4wla/pdb
• 関連するPDBエントリー: 2PHY 4WL9
• 分子名称: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (3 entities in total)
• 機能のキーワード: bluelight photoreceptor, time-resolved serial femtosecond crystallography, nanocrystals, microcrystals, signaling protein
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14052.73

構造登録者

Tenboer, J.,Schmidt, M. (登録日: 2014-10-06, 公開日: 2014-12-17, 最終更新日: 2023-09-27)

主引用文献

Tenboer, J.,Basu, S.,Zatsepin, N.,Pande, K.,Milathianaki, D.,Frank, M.,Hunter, M.,Boutet, S.,Williams, G.J.,Koglin, J.E.,Oberthuer, D.,Heymann, M.,Kupitz, C.,Conrad, C.,Coe, J.,Roy-Chowdhury, S.,Weierstall, U.,James, D.,Wang, D.,Grant, T.,Barty, A.,Yefanov, O.,Scales, J.,Gati, C.,Seuring, C.,Srajer, V.,Henning, R.,Schwander, P.,Fromme, R.,Ourmazd, A.,Moffat, K.,Van Thor, J.J.,Spence, J.C.,Fromme, P.,Chapman, H.N.,Schmidt, M.
Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.
Science, 346:1242-1246, 2014

PubMed Abstract: Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

PubMed: 25477465
DOI: 10.1126/science.1259357

実験手法

X-RAY DIFFRACTION (1.6 Å)