4WID

Crystal structure of the immediate-early 1 protein (IE1) at 2.31 angstrom (tetragonal form after crystal dehydration)

4WID の概要

• エントリーDOI: 10.2210/pdb4wid/pdb
• 関連するPDBエントリー: 4W1C
• 分子名称: RhUL123, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: antagonist, cytomegalovirus, viral protein
• 由来する生物種: Macacine herpesvirus 3 (Rhesus cytomegalovirus); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84751.58

構造登録者

Klingl, S.,Scherer, M.,Sevvana, M.,Muller, Y.A.,Stamminger, T. (登録日: 2014-09-25, 公開日: 2014-10-29, 最終更新日: 2024-05-01)

主引用文献

Scherer, M.,Klingl, S.,Sevvana, M.,Otto, V.,Schilling, E.M.,Stump, J.D.,Muller, R.,Reuter, N.,Sticht, H.,Muller, Y.A.,Stamminger, T.
Crystal Structure of Cytomegalovirus IE1 Protein Reveals Targeting of TRIM Family Member PML via Coiled-Coil Interactions.
Plos Pathog., 10:e1004512-e1004512, 2014

PubMed Abstract: PML nuclear bodies (PML-NBs) are enigmatic structures of the cell nucleus that act as key mediators of intrinsic immunity against viral pathogens. PML itself is a member of the E3-ligase TRIM family of proteins that regulates a variety of innate immune signaling pathways. Consequently, viruses have evolved effector proteins to modify PML-NBs; however, little is known concerning structure-function relationships of viral antagonists. The herpesvirus human cytomegalovirus (HCMV) expresses the abundant immediate-early protein IE1 that colocalizes with PML-NBs and induces their dispersal, which correlates with the antagonization of NB-mediated intrinsic immunity. Here, we delineate the molecular basis for this antagonization by presenting the first crystal structure for the evolutionary conserved primate cytomegalovirus IE1 proteins. We show that IE1 consists of a globular core (IE1CORE) flanked by intrinsically disordered regions. The 2.3 Å crystal structure of IE1CORE displays an all α-helical, femur-shaped fold, which lacks overall fold similarity with known protein structures, but shares secondary structure features recently observed in the coiled-coil domain of TRIM proteins. Yeast two-hybrid and coimmunoprecipitation experiments demonstrate that IE1CORE binds efficiently to the TRIM family member PML, and is able to induce PML deSUMOylation. Intriguingly, this results in the release of NB-associated proteins into the nucleoplasm, but not of PML itself. Importantly, we show that PML deSUMOylation by IE1CORE is sufficient to antagonize PML-NB-instituted intrinsic immunity. Moreover, co-immunoprecipitation experiments demonstrate that IE1CORE binds via the coiled-coil domain to PML and also interacts with TRIM5α We propose that IE1CORE sequesters PML and possibly other TRIM family members via structural mimicry using an extended binding surface formed by the coiled-coil region. This mode of interaction might render the antagonizing activity less susceptible to mutational escape.

PubMed: 25412268
DOI: 10.1371/journal.ppat.1004512

実験手法

X-RAY DIFFRACTION (2.31 Å)