4WF9

The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with telithromycin

Summary for 4WF9

• Entry DOI: 10.2210/pdb4wf9/pdb
• Related: 4WCE 4WFA 4WFB
• Descriptor: 23S ribosomal RNA, 50S ribosomal protein L15, 50S ribosomal protein L16, ... (33 entities in total)
• Functional Keywords: ribosome, rna, bacteria
• Biological source: Staphylococcus aureus subsp. aureus NCTC 8325; More
• Total number of polymer chains: 26
• Total formula weight: 1349619.79

Authors

Eyal, Z.,Matzov, D.,Krupkin, M.,Wekselman, I.,Zimmerman, E.,Rozenberg, H.,Bashan, A.,Yonath, A.E. (deposition date: 2014-09-14, release date: 2015-10-21, Last modification date: 2024-01-10)

Primary citation

Eyal, Z.,Matzov, D.,Krupkin, M.,Wekselman, I.,Paukner, S.,Zimmerman, E.,Rozenberg, H.,Bashan, A.,Yonath, A.
Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
Proc.Natl.Acad.Sci.USA, 112:E5805-E5814, 2015

PubMed Abstract: The emergence of bacterial multidrug resistance to antibiotics threatens to cause regression to the preantibiotic era. Here we present the crystal structure of the large ribosomal subunit from Staphylococcus aureus, a versatile Gram-positive aggressive pathogen, and its complexes with the known antibiotics linezolid and telithromycin, as well as with a new, highly potent pleuromutilin derivative, BC-3205. These crystal structures shed light on specific structural motifs of the S. aureus ribosome and the binding modes of the aforementioned antibiotics. Moreover, by analyzing the ribosome structure and comparing it with those of nonpathogenic bacterial models, we identified some unique internal and peripheral structural motifs that may be potential candidates for improving known antibiotics and for use in the design of selective antibiotic drugs against S. aureus.

PubMed: 26464510
DOI: 10.1073/pnas.1517952112

Experimental method

X-RAY DIFFRACTION (3.427 Å)