4WCE

The crystal structure of the large ribosomal subunit of Staphylococcus aureus

4WCE の概要

• エントリーDOI: 10.2210/pdb4wce/pdb
• 関連するPDBエントリー: 4WFA 4WFB 4WFI 6HMA
• 分子名称: 23S rRNA, 50S ribosomal protein L15, 50S ribosomal protein L16, ... (34 entities in total)
• 機能のキーワード: ribosome
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 1359223.53

構造登録者

Eyal, Z.,Matzov, D.,Krupkin, M.,Wekselman, I.,Zimmerman, E.,Rozenberg, H.,Bashan, A.,Yonath, A. (登録日: 2014-09-04, 公開日: 2015-10-21, 最終更新日: 2024-10-09)

主引用文献

Eyal, Z.,Matzov, D.,Krupkin, M.,Wekselman, I.,Paukner, S.,Zimmerman, E.,Rozenberg, H.,Bashan, A.,Yonath, A.
Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
Proc.Natl.Acad.Sci.USA, 112:E5805-E5814, 2015

PubMed Abstract: The emergence of bacterial multidrug resistance to antibiotics threatens to cause regression to the preantibiotic era. Here we present the crystal structure of the large ribosomal subunit from Staphylococcus aureus, a versatile Gram-positive aggressive pathogen, and its complexes with the known antibiotics linezolid and telithromycin, as well as with a new, highly potent pleuromutilin derivative, BC-3205. These crystal structures shed light on specific structural motifs of the S. aureus ribosome and the binding modes of the aforementioned antibiotics. Moreover, by analyzing the ribosome structure and comparing it with those of nonpathogenic bacterial models, we identified some unique internal and peripheral structural motifs that may be potential candidates for improving known antibiotics and for use in the design of selective antibiotic drugs against S. aureus.

PubMed: 26464510
DOI: 10.1073/pnas.1517952112

実験手法

X-RAY DIFFRACTION (3.526 Å)