4WAU

Crystal structure of CENP-M solved by native-SAD phasing

Summary for 4WAU

• Entry DOI: 10.2210/pdb4wau/pdb
• Related: 4P0T
• Descriptor: Centromere protein M (2 entities in total)
• Functional Keywords: native-sad, s-sad, sulfur-sad, sulfur sad, mitosis, kinetochore, ccan, g-protein, cell cycle
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus: Q9NSP4
• Total number of polymer chains: 2
• Total formula weight: 38490.89

Authors

Weinert, T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Wang, M. (deposition date: 2014-09-01, release date: 2014-12-10, Last modification date: 2024-05-08)

Primary citation

Weinert, T.,Olieric, V.,Waltersperger, S.,Panepucci, E.,Chen, L.,Zhang, H.,Zhou, D.,Rose, J.,Ebihara, A.,Kuramitsu, S.,Li, D.,Howe, N.,Schnapp, G.,Pautsch, A.,Bargsten, K.,Prota, A.E.,Surana, P.,Kottur, J.,Nair, D.T.,Basilico, F.,Cecatiello, V.,Pasqualato, S.,Boland, A.,Weichenrieder, O.,Wang, B.C.,Steinmetz, M.O.,Caffrey, M.,Wang, M.
Fast native-SAD phasing for routine macromolecular structure determination.
Nat.Methods, 12:131-133, 2015

PubMed Abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.

PubMed: 25506719
DOI: 10.1038/nmeth.3211

Experimental method

X-RAY DIFFRACTION (2.2 Å)