4W94
Crystal structure of cross-linked tetragonal hen egg white lysozyme soaked with 5mM [Ru(CO)3Cl2]2
Summary for 4W94
| Entry DOI | 10.2210/pdb4w94/pdb |
| Related | 4W96 |
| Descriptor | Lysozyme C, DIMETHYLFORMAMIDE, CHLORIDE ION, ... (7 entities in total) |
| Functional Keywords | hydrolase |
| Biological source | Gallus gallus (Chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 15824.01 |
| Authors | Tabe, H.,Fujita, K.,Abe, S.,Tsujimoto, M.,Kuchimaru, T.,Kizaka-Kondo, S.,Takano, M.,Kitagawa, S.,Ueno, T. (deposition date: 2014-08-27, release date: 2014-12-31, Last modification date: 2024-10-30) |
| Primary citation | Tabe, H.,Fujita, K.,Abe, S.,Tsujimoto, M.,Kuchimaru, T.,Kizaka-Kondoh, S.,Takano, M.,Kitagawa, S.,Ueno, T. Preparation of a Cross-Linked Porous Protein Crystal Containing Ru Carbonyl Complexes as a CO-Releasing Extracellular Scaffold Inorg.Chem., 54:215-220, 2015 Cited by PubMed Abstract: Protein crystals generally are stable solid protein assemblies. Certain protein crystals are suitable for use as nanovessels for immobilizing metal complexes. Here we report the preparation of ruthenium carbonyl-incorporated cross-linked hen egg white lysozyme crystals (Ru·CL-HEWL). Ru·CL-HEWL retains a Ru carbonyl moiety that can release CO, although a composite of Ru carbonyl-HEWL dissolved in buffer solution (Ru·HEWL) does not release CO. We found that treatment of cells with Ru·CL-HEWL significantly increased nuclear factor kappa B (NF-κB) activity as a cellular response to CO. These results demonstrate that Ru·CL-HEWL has potential for use as an artificial extracellular scaffold suitable for transport and release of a gas molecule. PubMed: 25494847DOI: 10.1021/ic502159x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.55 Å) |
Structure validation
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