4W94

Crystal structure of cross-linked tetragonal hen egg white lysozyme soaked with 5mM [Ru(CO)3Cl2]2

4W94 の概要

• エントリーDOI: 10.2210/pdb4w94/pdb
• 関連するPDBエントリー: 4W96
• 分子名称: Lysozyme C, DIMETHYLFORMAMIDE, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15824.01

構造登録者

Tabe, H.,Fujita, K.,Abe, S.,Tsujimoto, M.,Kuchimaru, T.,Kizaka-Kondo, S.,Takano, M.,Kitagawa, S.,Ueno, T. (登録日: 2014-08-27, 公開日: 2014-12-31, 最終更新日: 2024-10-30)

主引用文献

Tabe, H.,Fujita, K.,Abe, S.,Tsujimoto, M.,Kuchimaru, T.,Kizaka-Kondoh, S.,Takano, M.,Kitagawa, S.,Ueno, T.
Preparation of a Cross-Linked Porous Protein Crystal Containing Ru Carbonyl Complexes as a CO-Releasing Extracellular Scaffold
Inorg.Chem., 54:215-220, 2015

PubMed Abstract: Protein crystals generally are stable solid protein assemblies. Certain protein crystals are suitable for use as nanovessels for immobilizing metal complexes. Here we report the preparation of ruthenium carbonyl-incorporated cross-linked hen egg white lysozyme crystals (Ru·CL-HEWL). Ru·CL-HEWL retains a Ru carbonyl moiety that can release CO, although a composite of Ru carbonyl-HEWL dissolved in buffer solution (Ru·HEWL) does not release CO. We found that treatment of cells with Ru·CL-HEWL significantly increased nuclear factor kappa B (NF-κB) activity as a cellular response to CO. These results demonstrate that Ru·CL-HEWL has potential for use as an artificial extracellular scaffold suitable for transport and release of a gas molecule.

PubMed: 25494847
DOI: 10.1021/ic502159x

実験手法

X-RAY DIFFRACTION (1.55 Å)