4W6J

Crystal Structure of Full-Length Split GFP Mutant D117C Disulfide Dimer, P 31 2 1 Space Group

4W6J の概要

• エントリーDOI: 10.2210/pdb4w6j/pdb
• 関連するPDBエントリー: 4W69 4W6A 4W6B 4W6C 4W6D 4W6F 4W6G 4W6H 4W6J 4W6K 4W6L 4W6M 4W6N 4W6O 4W6P 4W6R 4W6S 4W6T 4W6U 4W72 4W73 4W74 4W75 4W76 4W77 4W7A 4W7C 4W7D 4W7E 4W7F 4W7R 4W7X
• 分子名称: fluorescent protein D117C, (4S)-2-METHYL-2,4-PENTANEDIOL, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: fluorescent protein, dimer, disulfide
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54400.39

構造登録者

Leibly, D.J.,Waldo, G.S.,Yeates, T.O. (登録日: 2014-08-20, 公開日: 2015-02-18, 最終更新日: 2023-11-15)

主引用文献

Leibly, D.J.,Arbing, M.A.,Pashkov, I.,DeVore, N.,Waldo, G.S.,Terwilliger, T.C.,Yeates, T.O.
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Structure, 23:1754-1768, 2015

PubMed Abstract: Applications ranging from synthetic biology to protein crystallization could be advanced by facile systems for connecting multiple proteins together in predefined spatial relationships. One approach to this goal is to engineer many distinct assembly forms of a single carrier protein or scaffold, to which other proteins of interest can then be readily attached. In this work we chose GFP as a scaffold and engineered many alternative oligomeric forms, driven by either specific disulfide bond formation or metal ion addition. We generated a wide range of spatial arrangements of GFP subunits from 11 different oligomeric variants, and determined their X-ray structures in a total of 33 distinct crystal forms. Some of the oligomeric GFP variants show geometric polymorphism depending on conditions, while others show considerable geometric rigidity. Potential future applications of this system are discussed.

PubMed: 26278175
DOI: 10.1016/j.str.2015.07.008

実験手法

X-RAY DIFFRACTION (1.702 Å)