4W4Q
Glucose isomerase structure determined by serial femtosecond crystallography at SACLA
Summary for 4W4Q
| Entry DOI | 10.2210/pdb4w4q/pdb |
| Descriptor | Xylose isomerase, CALCIUM ION (3 entities in total) |
| Functional Keywords | xfel, glucose isomerase, xylose isomerase, isomerase |
| Biological source | Streptomyces rubiginosus |
| Cellular location | Cytoplasm: P24300 |
| Total number of polymer chains | 1 |
| Total formula weight | 43323.38 |
| Authors | Nango, E.,Tanaka, T.,Sugahara, M.,Suzuki, M.,Iwata, S. (deposition date: 2014-08-15, release date: 2014-11-05, Last modification date: 2024-03-20) |
| Primary citation | Sugahara, M.,Mizohata, E.,Nango, E.,Suzuki, M.,Tanaka, T.,Masuda, T.,Tanaka, R.,Shimamura, T.,Tanaka, Y.,Suno, C.,Ihara, K.,Pan, D.,Kakinouchi, K.,Sugiyama, S.,Murata, M.,Inoue, T.,Tono, K.,Song, C.,Park, J.,Kameshima, T.,Hatsui, T.,Joti, Y.,Yabashi, M.,Iwata, S. Grease matrix as a versatile carrier of proteins for serial crystallography Nat.Methods, 12:61-63, 2015 Cited by PubMed Abstract: Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å. PubMed: 25384243DOI: 10.1038/nmeth.3172 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report
