4W2I
Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites
This is a non-PDB format compatible entry.
Summary for 4W2I
Entry DOI | 10.2210/pdb4w2i/pdb |
Descriptor | 16S Ribosomal RNA, 30S Ribosomal Protein S10, 30S Ribosomal Protein S11, ... (61 entities in total) |
Functional Keywords | negamycin, antibiotic, 70s ribosome, inhibition of translation, miscoding, translocation inhibitor, 30s subunit with negamycin, mrna, deacylated a-, p-, and e-site trnas of the 1st 70s ribosome in the asu, functional complex of bacterial 70s ribosome with antibiotic negamycin, 50s subunit of the 1st 70s ribosome in the asu, ribosome-antibiotic complex, ribosome/antibiotic |
Biological source | Escherichia coli More |
Total number of polymer chains | 112 |
Total formula weight | 4555287.71 |
Authors | Polikanov, Y.S.,Szal, T.,Jiang, F.,Gupta, P.,Matsuda, R.,Shiozuka, M.,Steitz, T.A.,Vazquez-Laslop, N.,Mankin, A.S. (deposition date: 2014-09-12, release date: 2014-10-15, Last modification date: 2023-12-27) |
Primary citation | Polikanov, Y.S.,Szal, T.,Jiang, F.,Gupta, P.,Matsuda, R.,Shiozuka, M.,Steitz, T.A.,Vazquez-Laslop, N.,Mankin, A.S. Negamycin Interferes with Decoding and Translocation by Simultaneous Interaction with rRNA and tRNA. Mol.Cell, 56:541-550, 2014 Cited by PubMed: 25306922DOI: 10.1016/j.molcel.2014.09.021 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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