Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (co-crystallized), mRNA and deacylated tRNA in the P site

This is a large structure.

Summary for 4W2H

Integrates4RBD 4RBE 4RBF 4RBG
Descriptor16S Ribosomal RNA, 30S Ribosomal Protein S2, 30S Ribosomal Protein S3, ... (61 entities in total)
Functional Keywordspactamycin, antibiotic, 70s ribosome, inhibition of translation, initiation inhibitor, 30s subunit with pactamycin (co-crystallized), mrna, deacylated p-site trna, and e-site trna acceptor stem of the 1st 70s ribosome in the asu, functional complex of bacterial 70s ribosome with antibiotic pactamycin (extended conformation), 50s subunit of the 1st 70s ribosome in the asu, ribosome-antibiotic complex, ribosome/antibiotic
Biological sourceEscherichia coli
Total number of polymer chains110
Total molecular weight4503125.04
Primary citation
Polikanov, Y.S.,Osterman, I.A.,Szal, T.,Tashlitsky, V.N.,Serebryakova, M.V.,Kusochek, P.,Bulkley, D.,Malanicheva, I.A.,Efimenko, T.A.,Efremenkova, O.V.,Konevega, A.L.,Shaw, K.J.,Bogdanov, A.A.,Rodnina, M.V.,Dontsova, O.A.,Mankin, A.S.,Steitz, T.A.,Sergiev, P.V.
Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.
Mol.Cell, 56:531-540, 2014
PubMed: 25306919 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2014.09.020
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.26311 1.4% 8.6% 7.7% 0.49MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution