4V9K

70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state.

これはPDB形式変換不可エントリーです。

4V9K の概要

• エントリーDOI: 10.2210/pdb4v9k/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000226
• 分子名称: 30S ribosomal protein S2, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (62 entities in total)
• 機能のキーワード: gdpnp, chimeric hybrid state ribosome, ribosome, translocation intermediate, ef-g, mrna, trna, ribosome-antibiotic complex, ribosome/antibiotic
• 由来する生物種: Thermus thermophilus; 詳細
• 細胞内の位置: Cytoplasm : Q72I01
• タンパク質・核酸の鎖数: 122
• 化学式量合計: 4569712.81

構造登録者

Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F. (登録日: 2013-04-24, 公開日: 2014-07-09, 最終更新日: 2023-12-06)

主引用文献

Zhou, J.,Lancaster, L.,Donohue, J.P.,Noller, H.F.
Crystal structures of EF-G-ribosome complexes trapped in intermediate states of translocation.
Science, 340:1236086-1236086, 2013

PubMed Abstract: Translocation of messenger and transfer RNA (mRNA and tRNA) through the ribosome is a crucial step in protein synthesis, whose mechanism is not yet understood. The crystal structures of three Thermus ribosome-tRNA-mRNA-EF-G complexes trapped with β,γ-imidoguanosine 5'-triphosphate (GDPNP) or fusidic acid reveal conformational changes occurring during intermediate states of translocation, including large-scale rotation of the 30S subunit head and body. In all complexes, the tRNA acceptor ends occupy the 50S subunit E site, while their anticodon stem loops move with the head of the 30S subunit to positions between the P and E sites, forming chimeric intermediate states. Two universally conserved bases of 16S ribosomal RNA that intercalate between bases of the mRNA may act as "pawls" of a translocational ratchet. These findings provide new insights into the molecular mechanism of ribosomal translocation.

PubMed: 23812722
DOI: 10.1126/science.1236086

実験手法

X-RAY DIFFRACTION (3.5 Å)