4V95

Crystal structure of YAEJ bound to the 70S ribosome

これはPDB形式変換不可エントリーです。

4V95 の概要

• エントリーDOI: 10.2210/pdb4v95/pdb
• 分子名称: 16S Ribosomal RNA, 30S Ribosomal Protein S10, 30S Ribosomal Protein S11, ... (58 entities in total)
• 機能のキーワード: ribosome, yaej, ribosome stalling, ribosome rescue, rescue factor, alternative rescue factor, arfb, release factor, rescue of stalled ribosomes
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 109
• 化学式量合計: 4470834.22

構造登録者

Gagnon, M.G.,Seetharaman, S.V.,Bulkley, D.P.,Steitz, T.A. (登録日: 2012-01-27, 公開日: 2014-07-09, 最終更新日: 2024-11-06)

主引用文献

Gagnon, M.G.,Seetharaman, S.V.,Bulkley, D.,Steitz, T.A.
Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.
Science, 335:1370-1372, 2012

PubMed Abstract: In bacteria, the hybrid transfer-messenger RNA (tmRNA) rescues ribosomes stalled on defective messenger RNAs (mRNAs). However, certain gram-negative bacteria have evolved proteins that are capable of rescuing stalled ribosomes in a tmRNA-independent manner. Here, we report a 3.2 angstrom-resolution crystal structure of the rescue factor YaeJ bound to the Thermus thermophilus 70S ribosome in complex with the initiator tRNA(i)(fMet) and a short mRNA. The structure reveals that the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes by binding in the mRNA entry channel downstream of the A site between the head and shoulder of the 30S subunit. This allows the N-terminal globular domain to sample different conformations, so that its conserved GGQ motif is optimally positioned to catalyze the hydrolysis of peptidyl-tRNA. This structure gives insights into the mechanism of YaeJ function and provides a basis for understanding how it rescues stalled ribosomes.

PubMed: 22422986
DOI: 10.1126/science.1217443

実験手法

X-RAY DIFFRACTION (3.2 Å)