4V7M

The structures of Capreomycin bound to the 70S ribosome.

これはPDB形式変換不可エントリーです。

4V7M の概要

• エントリーDOI: 10.2210/pdb4v7m/pdb
• 関連するPDBエントリー: 3KNH 3KNI 3KNJ 3KNK
• 関連するBIRD辞書のPRD_ID: PRD_000193
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (61 entities in total)
• 機能のキーワード: anti-tuberculosis antibiotic, 70s, ribosome, trna, mrna, tuberactinomycin, capreomycin, ribosome-antibiotic complex, ribosome/antibiotic
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 116
• 化学式量合計: 4566150.49

構造登録者

Stanley, R.E.,Blaha, G. (登録日: 2009-11-12, 公開日: 2014-07-09, 最終更新日: 2025-03-26)

主引用文献

Stanley, R.E.,Blaha, G.,Grodzicki, R.L.,Strickler, M.D.,Steitz, T.A.
The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome.
Nat.Struct.Mol.Biol., 17:289-293, 2010

PubMed Abstract: Viomycin and capreomycin belong to the tuberactinomycin family of antibiotics, which are among the most effective antibiotics against multidrug-resistant tuberculosis. Here we present two crystal structures of the 70S ribosome in complex with three tRNAs and bound to either viomycin or capreomycin at 3.3- and 3.5-A resolution, respectively. Both antibiotics bind to the same site on the ribosome, which lies at the interface between helix 44 of the small ribosomal subunit and helix 69 of the large ribosomal subunit. The structures of these complexes suggest that the tuberactinomycins inhibit translocation by stabilizing the tRNA in the A site in the pretranslocation state. In addition, these structures show that the tuberactinomycins bind adjacent to the binding sites for the paromomycin and hygromycin B antibiotics, which may enable the development of new derivatives of tuberactinomycins that are effective against drug-resistant strains.

PubMed: 20154709
DOI: 10.1038/nsmb.1755

実験手法

X-RAY DIFFRACTION (3.45 Å)