4V6V

Tetracycline resistance protein Tet(O) bound to the ribosome

This is a non-PDB format compatible entry.

Summary for 4V6V

• Entry DOI: 10.2210/pdb4v6v/pdb
• Related: 2I2U 2I2V
• EMDB information: 5562
• Descriptor: 30S ribosomal protein S10, 30S ribosomal protein S19, 30S ribosomal protein S2, ... (58 entities in total)
• Functional Keywords: 70s ribosome, antibiotic resistance, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 58
• Total formula weight: 2306325.93

Authors

Li, W.,Atkinson, G.C.,Thakor, N.S.,Allas, U.,Lu, C.,Chan, K.Y.,Tenson, T.,Schulten, K.,Wilson, K.S.,Hauryliuk, V.,Frank, J. (deposition date: 2013-02-25, release date: 2014-07-09, Last modification date: 2024-02-28)

Primary citation

Li, W.,Atkinson, G.C.,Thakor, N.S.,Allas, U.,Lu, C.C.,Chan, K.Y.,Tenson, T.,Schulten, K.,Wilson, K.S.,Hauryliuk, V.,Frank, J.
Mechanism of tetracycline resistance by ribosomal protection protein Tet(O).
Nat Commun, 4:1477-1477, 2013

PubMed Abstract: Tetracycline resistance protein Tet(O), which protects the bacterial ribosome from binding the antibiotic tetracycline, is a translational GTPase with significant similarity in both sequence and structure to the elongation factor EF-G. Here, we present an atomic model of the Tet(O)-bound 70S ribosome based on our cryo-electron microscopic reconstruction at 9.6-Å resolution. This atomic model allowed us to identify the Tet(O)-ribosome binding sites, which involve three characteristic loops in domain 4 of Tet(O). Replacements of the three amino-acid tips of these loops by a single glycine residue result in loss of Tet(O)-mediated tetracycline resistance. On the basis of these findings, the mechanism of Tet(O)-mediated tetracycline resistance can be explained in molecular detail.

PubMed: 23403578
DOI: 10.1038/ncomms2470

Experimental method

ELECTRON MICROSCOPY (9.8 Å)