4V6S

Structural characterization of mRNA-tRNA translocation intermediates (class 3 of the six classes)

これはPDB形式変換不可エントリーです。

4V6S の概要

• エントリーDOI: 10.2210/pdb4v6s/pdb
• 関連するPDBエントリー: 2I2U 2I2V 3J0T 3J0U 3J0V 3J0W 3J0X 3J0Y 3J0Z 3J10 3J12 3J14
• EMDBエントリー: 5359 5360 5361 5362 5363 5364
• 分子名称: 5S ribosomal RNA, 50S ribosomal protein L10, 50S ribosomal protein L11, ... (57 entities in total)
• 機能のキーワード: cryoelectron microscopy, molecular dynamics flexible fitting, ratchet-like rotation, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 57
• 化学式量合計: 2233671.70

構造登録者

Agirrezabala, X.,Liao, H.,Schreiner, E.,Fu, J.,Ortiz-Meoz, R.F.,Schulten, K.,Green, R.,Frank, J. (登録日: 2011-12-09, 公開日: 2014-07-09, 最終更新日: 2025-03-19)

主引用文献

Agirrezabala, X.,Liao, H.Y.,Schreiner, E.,Fu, J.,Ortiz-Meoz, R.F.,Schulten, K.,Green, R.,Frank, J.
Structural characterization of mRNA-tRNA translocation intermediates.
Proc.Natl.Acad.Sci.USA, 109:6094-6099, 2012

PubMed Abstract: Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation.

PubMed: 22467828
DOI: 10.1073/pnas.1201288109

実験手法

ELECTRON MICROSCOPY (13.1 Å)