4V6L

Structural insights into cognate vs. near-cognate discrimination during decoding.

これはPDB形式変換不可エントリーです。

4V6L の概要

• エントリーDOI: 10.2210/pdb4v6l/pdb
• EMDBエントリー: 1850
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S7, 30S ribosomal protein S8, ... (57 entities in total)
• 機能のキーワード: translation, ribosome, ternary complex, trna incorporation, cryoem, near-cognate
• 由来する生物種: Escherichia coli DH1; 詳細
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 2280361.06

構造登録者

Agirrezabala, X.,Schreiner, E.,Trabuco, L.G.,Lei, J.,Ortiz-Meoz, R.F.,Schulten, K.,Green, R.,Frank, J. (登録日: 2011-01-07, 公開日: 2014-07-09, 最終更新日: 2025-03-19)

主引用文献

Agirrezabala, X.,Schreiner, E.,Trabuco, L.G.,Lei, J.,Ortiz-Meoz, R.F.,Schulten, K.,Green, R.,Frank, J.
Structural insights into cognate versus near-cognate discrimination during decoding.
Embo J., 30:1497-1507, 2011

PubMed Abstract: The structural basis of the tRNA selection process is investigated by cryo-electron microscopy of ribosomes programmed with UGA codons and incubated with ternary complex (TC) containing the near-cognate Trp-tRNA(Trp) in the presence of kirromycin. Going through more than 350 000 images and employing image classification procedures, we find ∼8% in which the TC is bound to the ribosome. The reconstructed 3D map provides a means to characterize the arrangement of the near-cognate aa-tRNA with respect to elongation factor Tu (EF-Tu) and the ribosome, as well as the domain movements of the ribosome. One of the interesting findings is that near-cognate tRNA's acceptor stem region is flexible and CCA end becomes disordered. The data bring direct structural insights into the induced-fit mechanism of decoding by the ribosome, as the analysis of the interactions between small and large ribosomal subunit, aa-tRNA and EF-Tu and comparison with the cognate case (UGG codon) offers clues on how the conformational signals conveyed to the GTPase differ in the two cases.

PubMed: 21378755
DOI: 10.1038/emboj.2011.58

実験手法

ELECTRON MICROSCOPY (13.2 Å)