4V53

Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin.

これはPDB形式変換不可エントリーです。

4V53 の概要

• エントリーDOI: 10.2210/pdb4v53/pdb
• 関連するPDBエントリー: 2QBA 2QBB 2QBC
• 分子名称: 16S rRNA, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (56 entities in total)
• 機能のキーワード: rna-protein complex, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 104
• 化学式量合計: 4298846.85

構造登録者

Borovinskaya, M.A.,Pai, R.D.,Zhang, W.,Schuwirth, B.-S.,Holton, J.M.,Hirokawa, G.,Kaji, H.,Kaji, A.,Cate, J.H.D. (登録日: 2007-06-16, 公開日: 2014-07-09, 最終更新日: 2023-09-20)

主引用文献

Borovinskaya, M.A.,Pai, R.D.,Zhang, W.,Schuwirth, B.S.,Holton, J.M.,Hirokawa, G.,Kaji, H.,Kaji, A.,Cate, J.H.
Structural basis for aminoglycoside inhibition of bacterial ribosome recycling.
Nat.Struct.Mol.Biol., 14:727-732, 2007

PubMed Abstract: Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface. Aminoglycosides bind to H69 and completely restore the contacts between ribosomal subunits that are disrupted by RRF. These results provide a structural explanation for aminoglycoside inhibition of ribosome recycling.

PubMed: 17660832
DOI: 10.1038/nsmb1271

実験手法

X-RAY DIFFRACTION (3.54 Å)