4V47

Real space refined coordinates of the 30S and 50S subunits fitted into the low resolution cryo-EM map of the EF-G.GTP state of E. coli 70S ribosome

これはPDB形式変換不可エントリーです。

4V47 の概要

• エントリーDOI: 10.2210/pdb4v47/pdb
• 関連するPDBエントリー: 1GIX 1GIY 1IBL 1JJ2 1LNR 1M5G
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (46 entities in total)
• 機能のキーワード: ef-g.gtp-bound state, ratchet-like movement, real-space refinement, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 46
• 化学式量合計: 2075023.81

構造登録者

Gao, H.,Sengupta, J.,Valle, M.,Korostelev, A.,Eswar, N.,Stagg, S.M.,Van Roey, P.,Agrawal, R.K.,Harvey, S.T.,Sali, A.,Chapman, M.S.,Frank, J. (登録日: 2003-05-06, 公開日: 2014-07-09, 最終更新日: 2024-02-28)

主引用文献

Gao, H.,Sengupta, J.,Valle, M.,Korostelev, A.,Eswar, N.,Stagg, S.M.,Van Roey, P.,Agrawal, R.K.,Harvey, S.T.,Sali, A.,Chapman, M.S.,Frank, J.
Study of the structural dynamics of the E. coli 70S ribosome using real space refinement
Cell(Cambridge,Mass.), 113:789-801, 2003

PubMed Abstract: Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.

PubMed: 12809609
DOI: 10.1016/S0092-8674(03)00427-6

実験手法

ELECTRON MICROSCOPY (12.3 Å)