4V38

Apo-structure of alpha2,3-sialyltransferase variant 1 from Pasteurella dagmatis

4V38 の概要

• エントリーDOI: 10.2210/pdb4v38/pdb
• 関連するPDBエントリー: 4V2U 4V39 4V3B 4V3C
• 分子名称: SIALYLTRANSFERASE (2 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: PASTEURELLA DAGMATIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45703.06

構造登録者

Pavkov-Keller, T.,Schmoelzer, K.,Czabany, T.,Luley-Goedl, C.,Ribitsch, D.,Schwab, H.,Nidetzky, B.,Gruber, K. (登録日: 2014-10-17, 公開日: 2015-04-08, 最終更新日: 2024-01-10)

主引用文献

Schmoelzer, K.,Czabany, T.,Pavkov-Keller, T.,Luley-Goedl, C.,Ribitsch, D.,Schwab, H.,Gruber, K.,Nidetzky, B.
Complete Switch from Alpha2,3- to Alpha2,6-Regioselectivity in Pasteurella Dagmatis Beta-D-Galactoside Sialyltransferase by Active-Site Redesign
Chem.Commun.(Camb.), 51:3083-, 2015

PubMed Abstract: Structure-guided active-site redesign of a family GT-80 β-D-galactoside sialyltransferase (from Pasteurella dagmatis) to change enzyme regioselectivity from α-2,3 in the wild type to α-2,6 in a P7H-M117A double mutant is reported. Biochemical data for sialylation of lactose together with protein crystal structures demonstrate highly precise enzyme engineering.

PubMed: 25619424
DOI: 10.1039/C4CC09772F

実験手法

X-RAY DIFFRACTION (1.96 Å)