4V1Y

The structure of the hexameric atrazine chlorohydrolase, AtzA

4V1Y の概要

• エントリーDOI: 10.2210/pdb4v1y/pdb
• 関連するPDBエントリー: 4V1X
• 分子名称: ATRAZINE CHLOROHYDROLASE, FE (III) ION, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: hydrolase, bioremediation, protein evolution, dehalogenase
• 由来する生物種: PSEUDOMONAS SP. ADP
• 細胞内の位置: Cytoplasm : P72156
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 658882.23

構造登録者

Peat, T.S.,Newman, J.,Balotra, S.,Lucent, D.,Warden, A.C.,Scott, C. (登録日: 2014-10-04, 公開日: 2015-03-11, 最終更新日: 2024-01-10)

主引用文献

Peat, T.S.,Newman, J.,Balotra, S.,Lucent, D.,Warden, A.C.,Scott, C.
The Structure of the Hexameric Atrazine Chlorohydrolase Atza.
Acta Crystallogr.,Sect.D, 71:710-, 2015

PubMed Abstract: Atrazine chlorohydrolase (AtzA) was discovered and purified in the early 1990s from soil that had been exposed to the widely used herbicide atrazine. It was subsequently found that this enzyme catalyzes the first and necessary step in the breakdown of atrazine by the soil organism Pseudomonas sp. strain ADP. Although it has taken 20 years, a crystal structure of the full hexameric form of AtzA has now been obtained. AtzA is less well adapted to its physiological role (i.e. atrazine dechlorination) than the alternative metal-dependent atrazine chlorohydrolase (TrzN), with a substrate-binding pocket that is under considerable strain and for which the substrate is a poor fit.

PubMed: 25760618
DOI: 10.1107/S1399004715000619

実験手法

X-RAY DIFFRACTION (2.8 Å)