4UZM

Shotgun proteolysis: A practical application

4UZM の概要

• エントリーDOI: 10.2210/pdb4uzm/pdb
• 関連するPDBエントリー: 4UZW 4UZX
• NMR情報: BMRB: 25210
• 分子名称: PUTATIVE MEMBRANE PROTEIN IGAA HOMOLOG (1 entity in total)
• 機能のキーワード: structural protein
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P45800
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13435.93

構造登録者

Allen, M.D.,Bycroft, M.,Freund, S.M.V.,Christ, D. (登録日: 2014-09-05, 公開日: 2014-09-17, 最終更新日: 2024-05-15)

主引用文献

Allen, M.D.,Christie, M.,Jones, P.,Porebski, B.T.,Roome, B.,Freund, S.M.,Buckle, A.M.,Bycroft, M.,Christ, D.
Solution Structure of a Soluble Fragment Derived from a Membrane Protein by Shotgun Proteolysis.
Protein Eng.Des.Sel., 28:445-, 2015

PubMed Abstract: We have previously reported a phage display method for the identification of protein domains on a genome-wide scale (shotgun proteolysis). Here we present the solution structure of a fragment of the Escherichia coli membrane protein yrfF, as identified by shotgun proteolysis, and determined by NMR spectroscopy. Despite the absence of computational predictions, the fragment formed a well-defined beta-barrel structure, distantly falling within the OB-fold classification. Our results highlight the potential of high-throughput experimental approaches for the identification of protein domains for structural studies.

PubMed: 25877662
DOI: 10.1093/PROTEIN/GZV021

実験手法

SOLUTION NMR