4UYS

X-ray structure of the N-terminal domain of the flocculin Flo11 from Saccharomyces cerevisiae

4UYS の概要

• エントリーDOI: 10.2210/pdb4uys/pdb
• 関連するPDBエントリー: 4UYR 4UYT
• 分子名称: FLOCCULATION PROTEIN FLO11, SODIUM ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: cell adhesion, adhesin, flocculation, hydrophobic patches, homotypic binding
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Secreted, cell wall: P08640
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20748.55

構造登録者

Kraushaar, T.,Veelders, M.,Brueckner, S.,Rhinow, D.,Moesch, H.U.,Essen, L.O. (登録日: 2014-09-03, 公開日: 2015-08-12, 最終更新日: 2024-11-20)

主引用文献

Kraushaar, T.,Bruckner, S.,Veelders, M.,Rhinow, D.,Schreiner, F.,Birke, R.,Pagenstecher, A.,Mosch, H.,Essen, L.
Interactions by the Fungal Flo11 Adhesin Depend on a Fibronectin Type III-Like Adhesin Domain Girdled by Aromatic Bands.
Structure, 23:1005-, 2015

PubMed Abstract: Saccharomyces cerevisiae harbors a family of GPI-anchored cell wall proteins for interaction with its environment. The flocculin Flo11, a major representative of these fungal adhesins, confers formation of different types of multicellular structures such as biofilms, flors, or filaments. To understand these environment-dependent growth phenotypes on a molecular level, we solved the crystal structure of the N-terminal Flo11A domain at 0.89-Å resolution. Besides a hydrophobic apical region, the Flo11A domain consists of a β sandwich of the fibronectin type III domain (FN3). We further show that homophilic Flo11-Flo11 interactions and heterophilic Flo11-plastic interactions solely depend on the Flo11A domain and are strongly pH dependent. These functions of Flo11A involve an apical region with its surface-exposed aromatic band, which is accompanied by acidic stretches. Together with electron microscopic reconstructions of yeast cell-cell contact sites, our data suggest that Flo11 acts as a spacer-like, pH-sensitive adhesin that resembles a membrane-tethered hydrophobin.

PubMed: 25960408
DOI: 10.1016/J.STR.2015.03.021

実験手法

X-RAY DIFFRACTION (1.05 Å)