4UY3

Cytoplasmic domain of bacterial cell division protein ezra

4UY3 の概要

• エントリーDOI: 10.2210/pdb4uy3/pdb
• 関連するPDBエントリー: 4UXV
• 分子名称: SEPTATION RING FORMATION REGULATOR EZRA (1 entity in total)
• 機能のキーワード: structural protein, divisome, ftsz, cell wall, tubulin, spectrin, z-ring
• 由来する生物種: STAPHYLOCOCCUS AUREUS
• 細胞内の位置: Cell membrane ; Single-pass membrane protein : A7X3E7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23119.72

構造登録者

Cleverley, R.M.,Barrett, J.R.,Basle, A.,Khai-Bui, N.,Hewitt, L.,Solovyova, A.,Xu, Z.,Daniela, R.A.,Dixon, N.E.,Harry, E.J.,Oakley, A.J.,Vollmer, W.,Lewis, R.J. (登録日: 2014-08-28, 公開日: 2014-10-22, 最終更新日: 2024-05-01)

主引用文献

Cleverley, R.M.,Barrett, J.R.,Basle, A.,Bui, N.K.,Hewitt, L.,Solovyova, A.,Xu, Z.,Daniel, R.A.,Dixon, N.E.,Harry, E.J.,Oakley, A.J.,Vollmer, W.,Lewis, R.J.
Structure and Function of a Spectrin-Like Regulator of Bacterial Cytokinesis.
Nat.Commun., 5:5421-, 2014

PubMed Abstract: Bacterial cell division is facilitated by a molecular machine--the divisome--that assembles at mid-cell in dividing cells. The formation of the cytokinetic Z-ring by the tubulin homologue FtsZ is regulated by several factors, including the divisome component EzrA. Here we describe the structure of the 60-kDa cytoplasmic domain of EzrA, which comprises five linear repeats of an unusual triple helical bundle. The EzrA structure is bent into a semicircle, providing the protein with the potential to interact at both N- and C-termini with adjacent membrane-bound divisome components. We also identify at least two binding sites for FtsZ on EzrA and map regions of EzrA that are responsible for regulating FtsZ assembly. The individual repeats, and their linear organization, are homologous to the spectrin proteins that connect actin filaments to the membrane in eukaryotes, and we thus propose that EzrA is the founding member of the bacterial spectrin family.

PubMed: 25403286
DOI: 10.1038/NCOMMS6421

実験手法

X-RAY DIFFRACTION (2.6 Å)