4UVQ

PatG Domain of Unknown Function

4UVQ の概要

• エントリーDOI: 10.2210/pdb4uvq/pdb
• 分子名称: THIAZOLINE OXIDASE/SUBTILISIN-LIKE PROTEASE, ZINC ION (3 entities in total)
• 機能のキーワード: hydrolase, patellamides, cyanobactins, prochloron.
• 由来する生物種: PROCHLORON SP.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31386.59

構造登録者

Mann, G.,Koehnke, J.,Bent, A.F.,Graham, R.,Schwarz-Linek, U.,Naismith, J.H. (登録日: 2014-08-07, 公開日: 2014-09-17, 最終更新日: 2024-11-06)

主引用文献

Mann, G.,Koehnke, J.,Bent, A.F.,Graham, R.,Schwarz-Linek, U.,Naismith, J.H.
The Structure of the Cyanobactin Domain of Unknown Function from Patg in the Patellamide Gene Cluster
Acta Crystallogr.,Sect.F, 70:1597-, 2014

PubMed Abstract: Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological `toolkit' to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.

PubMed: 25484206
DOI: 10.1107/S2053230X1402425X

実験手法

X-RAY DIFFRACTION (1.724 Å)