4UU2

Ferulic acid decarboxylase from Enterobacter sp., single mutant

4UU2 の概要

• エントリーDOI: 10.2210/pdb4uu2/pdb
• 関連するPDBエントリー: 4UU3
• 分子名称: FERULIC ACID DECARBOXYLASE, PHOSPHATE ION, GLYCINE, ... (5 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: ENTEROBACTER SP.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38768.00

構造登録者

Hromic, A.,Pavkov-Keller, T.,Steinkellner, G.,Lyskowski, A.,Wuensch, C.,Gross, J.,Fuchs, M.,Fauland, K.,Glueck, S.M.,Faber, K.,Gruber, K. (登録日: 2014-07-24, 公開日: 2015-06-10, 最終更新日: 2024-05-01)

主引用文献

Wuensch, C.,Pavkov-Keller, T.,Steinkellner, G.,Gross, J.,Fuchs, M.,Hromic, A.,Lyskowski, A.,Fauland, K.,Gruber, K.,Glueck, S.M.,Faber, K.
Regioselective Enzymatic Beta-Carboxylation of Para-Hydroxy-Styrene Derivatives Catalyzed by Phenolic Acid Decarboxylases.
Adv. Synth. Catal., 357:1909-, 2015

PubMed Abstract: We report on a 'green' method for the utilization of carbon dioxide as C unit for the regioselective synthesis of ()-cinnamic acids regioselective enzymatic carboxylation of -hydroxystyrenes. Phenolic acid decarboxylases from bacterial sources catalyzed the β-carboxylation of -hydroxystyrene derivatives with excellent regio- and (/)-stereoselectivity by exclusively acting at the β-carbon atom of the C=C side chain to furnish the corresponding ()-cinnamic acid derivatives in up to 40% conversion at the expense of bicarbonate as carbon dioxide source. Studies on the substrate scope of this strategy are presented and a catalytic mechanism is proposed based on molecular modelling studies supported by mutagenesis of amino acid residues in the active site.

PubMed: 26190963
DOI: 10.1002/ADSC.201401028

実験手法

X-RAY DIFFRACTION (1.49 Å)