4USN

The structure of the immature HIV-1 capsid in intact virus particles at sub-nm resolution

4USN の概要

• エントリーDOI: 10.2210/pdb4usn/pdb
• EMDBエントリー: 2706
• 分子名称: P24 (1 entity in total)
• 機能のキーワード: viral protein, retrovirus, maturation, gag
• 由来する生物種: HUMAN IMMUNODEFICIENCY VIRUS 1 (HIV-1)
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 421701.97

構造登録者

Schur, F.K.M.,Hagen, W.J.H.,Rumlova, M.,Ruml, T.,Mueller, B.,Kraeusslich, H.-G.,Briggs, J.A.G. (登録日: 2014-07-11, 公開日: 2014-11-05, 最終更新日: 2024-05-08)

主引用文献

Schur, F.K.M.,Hagen, W.J.H.,Rumlova, M.,Ruml, T.,Muller, B.,Krausslich, H.,Briggs, J.A.G.
Structure of the Immature HIV-1 Capsid in Intact Virus Particles at 8.8 A Resolution.
Nature, 517:505-, 2015

PubMed Abstract: Human immunodeficiency virus type 1 (HIV-1) assembly proceeds in two stages. First, the 55 kilodalton viral Gag polyprotein assembles into a hexameric protein lattice at the plasma membrane of the infected cell, inducing budding and release of an immature particle. Second, Gag is cleaved by the viral protease, leading to internal rearrangement of the virus into the mature, infectious form. Immature and mature HIV-1 particles are heterogeneous in size and morphology, preventing high-resolution analysis of their protein arrangement in situ by conventional structural biology methods. Here we apply cryo-electron tomography and sub-tomogram averaging methods to resolve the structure of the capsid lattice within intact immature HIV-1 particles at subnanometre resolution, allowing unambiguous positioning of all α-helices. The resulting model reveals tertiary and quaternary structural interactions that mediate HIV-1 assembly. Strikingly, these interactions differ from those predicted by the current model based on in vitro-assembled arrays of Gag-derived proteins from Mason-Pfizer monkey virus. To validate this difference, we solve the structure of the capsid lattice within intact immature Mason-Pfizer monkey virus particles. Comparison with the immature HIV-1 structure reveals that retroviral capsid proteins, while having conserved tertiary structures, adopt different quaternary arrangements during virus assembly. The approach demonstrated here should be applicable to determine structures of other proteins at subnanometre resolution within heterogeneous environments.

PubMed: 25363765
DOI: 10.1038/NATURE13838

実験手法

ELECTRON MICROSCOPY (8.8 Å)