4US5

Crystal Structure of apo-MsnO8

4US5 の概要

• エントリーDOI: 10.2210/pdb4us5/pdb
• 分子名称: LUCIFERASE-LIKE MONOOXYGENASE, SODIUM ION, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (8 entities in total)
• 機能のキーワード: oxidoreductase, epoxide-formation, mensacarcin
• 由来する生物種: STREPTOMYCES BOTTROPENSIS; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 152957.86

構造登録者

Maier, S.,Pflueger, T.,Loesgen, S.,Asmus, K.,Broetz, E.,Paululat, T.,Zeeck, A.,Andrade, S.,Bechthold, A. (登録日: 2014-07-03, 公開日: 2014-07-16, 最終更新日: 2024-11-20)

主引用文献

Maier, S.,Pflueger, T.,Loesgen, S.,Asmus, K.,Broetz, E.,Paululat, T.,Zeeck, A.,Andrade, S.,Bechthold, A.
Insights Into the Bioactivity of Mensacarcin and Epoxide Formation by Msno8.
Chembiochem, 15:749-, 2014

PubMed Abstract: Mensacarcin, a potential antitumour drug, is produced by Streptomyces bottropensis. The structure consists of a three-membered ring system with many oxygen atoms. Of vital importance in this context is an epoxy moiety in the side chain of mensacarcin. Our studies with different mensacarcin derivatives have demonstrated that this epoxy group is primarily responsible for the cytotoxic effect of mensacarcin. In order to obtain further information about this epoxy moiety, inactivation experiments in the gene cluster were carried out to identify the epoxy-forming enzyme. Therefore the cosmid cos2, which covers almost the complete type II polyketide synthase (PKS) gene cluster, was heterologously expressed in Streptomyces albus. This led to production of didesmethylmensacarcin, due to the fact that methyltransferase genes are missing in the cosmid. Further gene inactivation experiments on this cosmid showed that MsnO8, a luciferase-like monooxygenase, introduces the epoxy group at the end of the biosynthesis of mensacarcin. In addition, the protein MsnO8 was purified, and its crystal structure was determined to a resolution of 1.80 Å.

PubMed: 24554499
DOI: 10.1002/CBIC.201300704

実験手法

X-RAY DIFFRACTION (1.8 Å)