4UQ8

Electron cryo-microscopy of bovine Complex I

4UQ8 の概要

• エントリーDOI: 10.2210/pdb4uq8/pdb
• EMDBエントリー: 2676
• 分子名称: NADH UBIQUINONE OXIDOREDUCTASE CHAIN 3, NADH UBIQUINONE OXIDOREDUCTASE CHAIN 6, NADH UBIQUINONE OXIDOREDUCTASE CHAIN 4L, ... (45 entities in total)
• 機能のキーワード: nadh dehydrogenase, respiratory complex, oxidoreductase
• 由来する生物種: BOS TAURUS (CATTLE); 詳細
• タンパク質・核酸の鎖数: 49
• 化学式量合計: 517635.08

構造登録者

Vinothkumar, K.R.,Zhu, J.,Hirst, J. (登録日: 2014-06-21, 公開日: 2014-10-01, 最終更新日: 2024-05-08)

主引用文献

Vinothkumar, K.R.,Zhu, J.,Hirst, J.
Architecture of Mammalian Respiratory Complex I.
Nature, 515:80-, 2014

PubMed Abstract: Complex I (NADH:ubiquinone oxidoreductase) is essential for oxidative phosphorylation in mammalian mitochondria. It couples electron transfer from NADH to ubiquinone with proton translocation across the energy-transducing inner membrane, providing electrons for respiration and driving ATP synthesis. Mammalian complex I contains 44 different nuclear- and mitochondrial-encoded subunits, with a combined mass of 1 MDa. The 14 conserved 'core' subunits have been structurally defined in the minimal, bacterial complex, but the structures and arrangement of the 30 'supernumerary' subunits are unknown. Here we describe a 5 Å resolution structure of complex I from Bos taurus heart mitochondria, a close relative of the human enzyme, determined by single-particle electron cryo-microscopy. We present the structures of the mammalian core subunits that contain eight iron-sulphur clusters and 60 transmembrane helices, identify 18 supernumerary transmembrane helices, and assign and model 14 supernumerary subunits. Thus, we considerably advance knowledge of the structure of mammalian complex I and the architecture of its supernumerary ensemble around the core domains. Our structure provides insights into the roles of the supernumerary subunits in regulation, assembly and homeostasis, and a basis for understanding the effects of mutations that cause a diverse range of human diseases.

PubMed: 25209663
DOI: 10.1038/NATURE13686

実験手法

ELECTRON MICROSCOPY (4.95 Å)