4UPU

Crystal structure of IP3 3-K calmodulin binding region in complex with Calmodulin

4UPU の概要

• エントリーDOI: 10.2210/pdb4upu/pdb
• 分子名称: CALMODULIN, INOSITOL-TRISPHOSPHATE 3-KINASE A, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton, spindle : P62158
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20041.35

構造登録者

Franco-Echevarria, E.,Banos-Sanz, J.I.,Monterroso, B.,Round, A.,Sanz-Aparicio, J.,Gonzalez, B. (登録日: 2014-06-18, 公開日: 2014-08-20, 最終更新日: 2024-01-10)

主引用文献

Franco-Echevarria, E.,Banos-Sanz, J.I.,Monterroso, B.,Round, A.,Sanz-Aparicio, J.,Gonzalez, B.
A New Calmodulin Binding Motif for Inositol 1,4,5-Trisphosphate 3-Kinase Regulation.
Biochem.J., 463:319-, 2014

PubMed Abstract: IP3-3K [Ins(1,4,5)P3 3-kinase] is a key enzyme that catalyses the synthesis of Ins(1,3,4,5)P4, using Ins(1,4,5)P3 and ATP as substrates. Both inositides, substrate and product, present crucial roles in the cell. Ins(1,4,5)P3 is a key point in Ca2+ metabolism that promotes Ca2+ release from intracellular stores and together with Ins(1,3,4,5)P4 regulates Ca2+ homoeostasis. In addition, Ins(1,3,4,5)P4 is involved in immune cell development. It has been proved that Ca2+/CaM (calmodulin) regulates the activity of IP3-3K, via direct interaction between both enzymes. Although we have extensive structural knowledge of the kinase domains of the three IP3-3K isoforms, no structural information is available about the interaction between IP3-3K and Ca2+/CaM. In the present paper we describe the crystal structure of the complex between human Ca2+/CaM and the CaM-binding region of human IP3-3K isoform A (residues 158-183) and propose a model for a complex including the kinase domain. The structure obtained allowed us to identify all of the key residues involved in the interaction, which have been evaluated by site-directed mutagenesis, pull-down and fluorescence anisotropy experiments. The results allowed the identification of a new CaM-binding motif, expanding our knowledge about how CaM interacts with its partners.

PubMed: 25101901
DOI: 10.1042/BJ20140757

実験手法

X-RAY DIFFRACTION (2.34 Å)