4UPK

Phosphonate monoester hydrolase SpPMH from Silicibacter pomeroyi

4UPK の概要

• エントリーDOI: 10.2210/pdb4upk/pdb
• 関連するPDBエントリー: 4UPI 4UPL
• 分子名称: PHOSPHONATE MONOESTER HYDROLASE (2 entities in total)
• 機能のキーワード: hydrolase, alakaline phosphatase superfamily, promiscuity
• 由来する生物種: RUEGERIA POMEROYI
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 180630.84

構造登録者

Valkov, E.,van Loo, B.,Hollfelder, F.,Hyvonen, M. (登録日: 2014-06-17, 公開日: 2015-07-01, 最終更新日: 2024-01-10)

主引用文献

van Loo, B.,Bayer, C.D.,Fischer, G.,Jonas, S.,Valkov, E.,Mohamed, M.F.,Vorobieva, A.,Dutruel, C.,Hyvonen, M.,Hollfelder, F.
Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily.
J.Am.Chem.Soc., 141:370-387, 2019

PubMed Abstract: Highly proficient, promiscuous enzymes can be springboards for functional evolution, able to avoid loss of function during adaptation by their capacity to promote multiple reactions. We employ a systematic comparative study of structure, sequence, and substrate specificity to track the evolution of specificity and reactivity between promiscuous members of clades of the alkaline phosphatase (AP) superfamily. Construction of a phylogenetic tree of protein sequences maps out the likely transition zone between arylsulfatases (ASs) and phosphonate monoester hydrolases (PMHs). Kinetic analysis shows that all enzymes characterized have four chemically distinct phospho- and sulfoesterase activities, with rate accelerations ranging from 10- to 10-fold for their primary and 10- to 10-fold for their promiscuous reactions, suggesting that catalytic promiscuity is widespread in the AP-superfamily. This functional characterization and crystallography reveal a novel class of ASs that is so similar in sequence to known PMHs that it had not been recognized as having diverged in function. Based on analysis of snapshots of catalytic promiscuity "in transition", we develop possible models that would allow functional evolution and determine scenarios for trade-off between multiple activities. For the new ASs, we observe largely invariant substrate specificity that would facilitate the transition from ASs to PMHs via trade-off-free molecular exaptation, that is, evolution without initial loss of primary activity and specificity toward the original substrate. This ability to bypass low activity generalists provides a molecular solution to avoid adaptive conflict.

PubMed: 30497259
DOI: 10.1021/jacs.8b10290

実験手法

X-RAY DIFFRACTION (2.24 Å)