4UOS
Thermodynamic hyperstability in parametrically designed helical bundles
Summary for 4UOS
| Entry DOI | 10.2210/pdb4uos/pdb |
| Related | 4UOT |
| Descriptor | DESIGNED HELICAL BUNDLE (2 entities in total) |
| Functional Keywords | de novo protein, protein design, thermodynamic hyperstability helical bundle |
| Biological source | SYNTHETIC CONSTRUCT |
| Total number of polymer chains | 1 |
| Total formula weight | 22439.83 |
| Authors | Oberdorfer, G.,Huang, P.,Pei, X.Y.,Xu, C.,Gonen, T.,Nannenga, B.,DiMaio, D.,Rogers, J.,Luisi, B.F.,Baker, D. (deposition date: 2014-06-09, release date: 2014-11-05, Last modification date: 2024-05-08) |
| Primary citation | Huang, P.,Oberdorfer, G.,Xu, C.,Pei, X.Y.,Nannenga, B.L.,Rogers, J.M.,Dimaio, F.,Gonen, T.,Luisi, B.,Baker, D. High Thermodynamic Stability of Parametrically Designed Helical Bundles Science, 346:481-, 2014 Cited by PubMed Abstract: We describe a procedure for designing proteins with backbones produced by varying the parameters in the Crick coiled coil-generating equations. Combinatorial design calculations identify low-energy sequences for alternative helix supercoil arrangements, and the helices in the lowest-energy arrangements are connected by loop building. We design an antiparallel monomeric untwisted three-helix bundle with 80-residue helices, an antiparallel monomeric right-handed four-helix bundle, and a pentameric parallel left-handed five-helix bundle. The designed proteins are extremely stable (extrapolated ΔGfold > 60 kilocalories per mole), and their crystal structures are close to those of the design models with nearly identical core packing between the helices. The approach enables the custom design of hyperstable proteins with fine-tuned geometries for a wide range of applications. PubMed: 25342806DOI: 10.1126/SCIENCE.1257481 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.63 Å) |
Structure validation
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