4UM8

Crystal structure of alpha V beta 6

4UM8 の概要

• エントリーDOI: 10.2210/pdb4um8/pdb
• 関連するPDBエントリー: 4UM9
• 分子名称: INTEGRIN ALPHA-V, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CHLORIDE ION, ... (18 entities in total)
• 機能のキーワード: immune system, cell surface receptor
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 334023.26

構造登録者

Dong, X.,Springer, T.A. (登録日: 2014-05-15, 公開日: 2014-11-12, 最終更新日: 2024-10-23)

主引用文献

Dong, X.,Hudson, N.E.,Lu, C.,Springer, T.A.
Structural Determinants of Integrin Beta-Subunit Specificity for Latent Tgf-Beta
Nat.Struct.Mol.Biol., 21:1091-, 2014

PubMed Abstract: Eight integrin α-β heterodimers recognize ligands with an Arg-Gly-Asp (RGD) motif. However, the structural mechanism by which integrins differentiate among extracellular proteins with RGD motifs is not understood. Here, crystal structures, mutations and peptide-affinity measurements show that αVβ6 binds with high affinity to a RGDLXXL/I motif within the prodomains of TGF-β1 and TGF-β3. The LXXL/I motif forms an amphipathic α-helix that binds in a hydrophobic pocket in the β6 subunit. Elucidation of the basis for ligand binding specificity by the integrin β subunit reveals contributions by three different βI-domain loops, which we designate specificity-determining loops (SDLs) 1, 2 and 3. Variation in a pair of single key residues in SDL1 and SDL3 correlates with the variation of the entire β subunit in integrin evolution, thus suggesting a paradigmatic role in overall β-subunit function.

PubMed: 25383667
DOI: 10.1038/NSMB.2905

実験手法

X-RAY DIFFRACTION (2.852 Å)