4UJC

mammalian 80S HCV-IRES initiation complex with eIF5B POST-like state

これはPDB形式変換不可エントリーです。

4UJC の概要

• エントリーDOI: 10.2210/pdb4ujc/pdb
• 関連するPDBエントリー: 4UPW 4UPX 4UPY
• EMDBエントリー: 2683
• 分子名称: TRNA, 60S RIBOSOMAL PROTEIN L5, 60S RIBOSOMAL PROTEIN L6, ... (85 entities in total)
• 機能のキーワード: ribosome, translation initiation, hepatitis c virus internal ribosome entry site, eukaryotic initiation factor 5b
• 由来する生物種: ORYCTOLAGUS CUNICULUS (RABBIT); 詳細
• タンパク質・核酸の鎖数: 83
• 化学式量合計: 4041153.40

構造登録者

Yamamoto, H.,Unbehaun, A.,Loerke, J.,Behrmann, E.,Marianne, C.,Burger, J.,Mielke, T.,Spahn, C.M.T. (登録日: 2014-06-18, 公開日: 2014-07-30, 最終更新日: 2024-11-13)

主引用文献

Yamamoto, H.,Unbehaun, A.,Loerke, J.,Behrmann, E.,Collier, M.,Burger, J.,Mielke, T.,Spahn, C.M.T.
Structure of the Mammalian 80S Initiation Complex with Initiation Factor 5B on Hcv-Ires RNA.
Nat.Struct.Mol.Biol., 21:721-, 2014

PubMed Abstract: The universally conserved eukaryotic initiation factor (eIF) 5B, a translational GTPase, is essential for canonical translation initiation. It is also required for initiation facilitated by the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) RNA. eIF5B promotes joining of 60S ribosomal subunits to 40S ribosomal subunits bound by initiator tRNA (Met-tRNAi(Met)). However, the exact molecular mechanism by which eIF5B acts has not been established. Here we present cryo-EM reconstructions of the mammalian 80S-HCV-IRES-Met-tRNAi(Met)-eIF5B-GMPPNP complex. We obtained two substates distinguished by the rotational state of the ribosomal subunits and the configuration of initiator tRNA in the peptidyl (P) site. Accordingly, a combination of conformational changes in the 80S ribosome and in initiator tRNA facilitates binding of the Met-tRNAi(Met) to the 60S P site and redefines the role of eIF5B as a tRNA-reorientation factor.

PubMed: 25064512
DOI: 10.1038/NSMB.2859

実験手法

ELECTRON MICROSCOPY (9.5 Å)