4UHV
The structure of VgrG1, the needle tip of the bacterial Type VI Secretion System
Summary for 4UHV
| Entry DOI | 10.2210/pdb4uhv/pdb |
| Descriptor | VGRG1, VALINE-GLYCINE REPEAT PROTEIN G1, CHLORIDE ION, SODIUM ION, ... (4 entities in total) |
| Functional Keywords | structural protein, vgrg1, virulence, toxin, effector, puncturing device, spike, t6ss, p. aeruginosa |
| Biological source | PSEUDOMONAS AERUGINOSA |
| Total number of polymer chains | 2 |
| Total formula weight | 148829.29 |
| Authors | Spinola-Amilibia, M.,Davo-Siguero, I.,Ruiz, F.M.,Santillana, E.,Medrano, F.J.,Romero, A. (deposition date: 2015-03-25, release date: 2016-01-27, Last modification date: 2024-01-10) |
| Primary citation | Spinola-Amilibia, M.,Davo-Siguero, I.,Ruiz, F.M.,Santillana, E.,Medrano, F.J.,Romero, A. The Structure of Vgrg1 from Pseudomonas Aeruginosa, the Needle Tip of the Bacterial Type Vi Secretion System Acta Crystallogr.,Sect.D, 72:34-, 2016 Cited by PubMed Abstract: When 300 kV cryo-EM images at Scherzer focus are acquired from ∼ 100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. This procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals. PubMed: 26894532DOI: 10.1107/S205979831502149X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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